生物制造 經典文獻導讀|2017年11月14日

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Tissue-engineered cornea constructed with compressed collagen and laser-perforated electrospun mat.

使用壓縮膠原以及激光打孔的電紡膜制備新型的組織工程角膜

BinKong, Wei Sun, Guoshi Chen, Song Tang, Ming Li, Zengwu Shao & Shengli Mi

https://www.nature.com/articles/s41598-017-01072-0

Abstract

While Plastic Compressed(PC) collagen technique is often used to fabricate bioengineered constructs, PCcollagen gels are too weak to be sutured or conveniently handled for clinicalapplications. To overcome this limitation, electrospun poly (lactic-co-glycolide)(PLGA) mats, which have excellent biocompatibility and mechanical properties,were combined with PC collagen to fabricate sandwich-like hybrid constructs. Bylaser-perforating holes with different sizes and spacings in the electrospunmats to regulate the mechanical properties and light transmittance of thehybrid constructs, we produced hybrid constructs with properties very suitableto apply in corneal tissue engineering.

(導讀:劉睿)壓縮膠原是一種常用的制造作組織工程支架的材料,但是對于人工角膜來說,壓縮膠原的力學性能無法滿足臨床的縫合手術的需求。本研究采用電紡的方法,將具有優異生物相容性和力學性能的PLGA材料制成電紡膜,使用激光對電紡膜打孔,再同壓縮膠原結合制成三明治式結構的角膜支架。通過調節激光打孔形成的孔徑大小,來對角膜支架的力學性能和透光性進行優化,最后得到適用于人工角膜的組織工程支架。


Carcinoma-associated fibroblasts promotedtumor spheroid invasion on a microfluidic 3D co-culture device

?在微流控3D共培養裝置上腫瘤相關成纖維細胞促進球狀腫瘤體的

Tingjiao Liu,?Bingcheng Lina?and Jianhua Qina.? LabChip, 2010, 10, 1671–1677

http://pubs.rsc.org/-/content/articlehtml/2010/lc/c000022a

Abstract

Carcinoma-associated fibroblasts (CAFs) area key determinant in malignant progression of cancer?and represent an important target forcancer therapies. In this work, we present a microfluidic-based 3D co-culturedevice to reconstruct an in vitro tumor microenvironment and firstlyinvestigate the effect of CAFs on cancer cell invasion in 3D matrix. Thisdevice is composed of six co-culture units, which enable parallel co-cultureassays to be run in the presence of 3D extracellular matrix. Salivary glandadenoid cystic carcinoma (ACC) cells and CAFs embedded in matrix wereco-cultured without direct contact on the device. Communication between ACCcells and CAFs could be established via medium diffused in matrix. It wasobserved that CAFs promoted ACC cell invasion in 3D matrix in a spheroidfashion, indicating that CAFs play a critical role in cancer invasion. Wefurther demonstrated the effect of MMP inhibitor as an agent against CAF-promotedcancer invasion. This co-culture device reproducibly reflected the in vivogrowth and invasion pattern of ACC and recreated the stroma-regulated ACCinvasion. Thus, it provides a suitable platform for elucidating the mechanismof CAF-regulated cancer invasion and discovering anti-invasion drugs in a welldefined 3D environment.

(導讀:劉兆宇)腫瘤相關成纖維細胞 CAFs是腫瘤細胞遷移進程的決定因素,因而成為了癌癥治療的重要目標。本文作者提出了一個基于微流控技術的三維共培養裝置,在體外重建了腫瘤微環境,并首先在三維結構內研究了CAFs對癌細胞侵襲能力的影響。該裝置由六個共培養單元組成,每個共培養單元中細胞平行培養于共培養單元內的相鄰流道。共同培養的細胞包括唾液腺腺樣囊性癌(ACC)細胞和包埋在膠原中的CAF而不使兩種細胞直接接觸。 ACC細胞和CAF之間的聯系可以通過在基質中擴散的介質來建立。可以觀察到CAFs促進了3D基質中的ACC細胞以球體的形式侵入,表明CAF在癌癥侵襲中起關鍵作用。本文進一步證實MMP抑制劑可以起到抗CAF促進的癌癥侵襲的作用。這種共培養裝置可再現地反映了ACC的體內生長和侵襲模式,并再現了癌旁調節ACC侵襲的機制。因此,它為闡明CAF調控癌癥侵襲的機制以及在明確的3D環境中發現抗侵入藥物提供了一個合適的平臺。




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